FastQCFastQC Report
Tue 7 Jan 2020
HV3K3BGXC_n01_35_NxW_C12-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3K3BGXC_n01_35_NxW_C12-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7041083
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT6900.012.6815841
ATCGCAC9250.012.1082568
GCGGGGT3205.0276467E-612.0311434
GGCGCGC3003.4396675E-511.66647738
CGGGAAT22650.011.4352621
TGCGGGG4105.781858E-711.097774533
GCGCGAT3501.36146955E-511.0003681
CCGGCAT10700.010.467641
CCCGTTT16450.010.2131071
CGCGTAT5653.4511686E-89.9118361
CGGGGCT5301.4429679E-79.9059921
CGGGCGT3259.2745374E-49.6926321
GCCCGAT4005.9293445E-59.6253211
GCGGGAT11650.09.6140561
CGCGAAC7352.5283953E-109.5241281
CGCCCAT12900.09.4964431
TCGCACA11550.09.3940539
GGGTCGC4905.6755E-69.28543247
GCCGGAT15900.09.2455921
CCGCGCA3150.00780505558.88912316
Produced by FastQC (version 0.11.5)