FastQCFastQC Report
Tue 7 Jan 2020
HV3K3BGXC_n01_34_NxW_C12-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3K3BGXC_n01_34_NxW_C12-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7666780
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCCCT6100.012.0507631
CGCGCGG3307.0854803E-611.66652536
CCGGCAT12700.09.9225631
GCGGAAT16300.09.8785981
CGGGCGC3955.167465E-59.74690815
CCGGGAT20300.09.6564411
GTCGGAT29900.09.4828511
GCCGGAT17400.09.4552661
CGGGCAT8657.2759576E-129.3075811
CCCACAT17500.09.2012091
CGGGGAT13850.09.0986681
CGCCAAT14700.09.0488071
CCCCTAT5452.2374534E-68.9920073
GCCAGAT22600.08.983481
CGGGGGT3600.00235472928.751151
CCCCCTA4401.6773122E-48.74960849
CCGACAT18500.08.7038461
GGAATAT36950.08.52615451
CGCGTAT6201.3932895E-68.4688551
TCGCGCG4552.4105427E-48.46143535
Produced by FastQC (version 0.11.5)