FastQCFastQC Report
Tue 7 Jan 2020
HV3K3BGXC_n01_33_NxW_C11-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3K3BGXC_n01_33_NxW_C11-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4674781
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAT2000.002781750212.2510931
CCCCACT5451.0913936E-1112.2029241
CCCGTAT3205.0211165E-612.0323231
CATGCGC3802.1619235E-711.97321665
GCCAGAT13450.011.9713291
CGCCCAT8500.011.9422421
CGCGCAT2651.4055661E-411.8878531
CGGGGAT6107.2759576E-1211.4764341
CGCCCCT2150.004777027811.3963661
CGCCCTT4702.4750989E-811.1712091
CGGGAAT13350.011.0122181
TGCGCTA4652.5643203E-710.53722267
CCCACAT11200.010.313421
CGGGCAT5502.2711902E-810.1827271
CGCGCAC3208.049849E-49.84336540
CACGACT6908.112693E-109.6385411
CGGGAAC16350.09.6338872
CGCGGAG6201.4113539E-89.59763051
ACATGCG5855.912807E-89.57227564
CCGGAAT20900.09.5463071