FastQCFastQC Report
Tue 7 Jan 2020
HV3K3BGXC_n01_32_NxW_C11-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3K3BGXC_n01_32_NxW_C11-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5747279
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAT3254.239755E-712.9243811
CCCCGAT4204.6784407E-912.5012621
CGCCCTT5950.012.3541881
CGCGTAT4308.688585E-811.3964991
CGCCCAT11050.011.0870921
CGCGGAT8300.010.9649631
CGCACAT5451.6971171E-910.9185331
GCGCCCG2650.001871570610.56618526
GCGGACC3106.0058676E-410.1623161
CGCCCCT3106.0058676E-410.1623161
TGCGCTA5906.2809704E-910.08444767
GGCGCCC2850.00342319939.82469825
ACGCGGG3300.0010671519.54517247
TCGCACA9300.09.408579
CCGGGAT15650.09.3939191
CGGGCAT6352.0845619E-89.3710241
GCCAGAT15700.09.3640021
CGGACAT7503.7107384E-109.3342751
GGGCCCT5653.6571146E-79.2920048
CCCGTAT5704.1485873E-79.2114551
Produced by FastQC (version 0.11.5)