FastQCFastQC Report
Tue 7 Jan 2020
HV3K3BGXC_n01_31_NxW_C11-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3K3BGXC_n01_31_NxW_C11-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5615684
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGCT3254.2427018E-712.9236261
CGCCAAT14100.011.91541
CGCACAA10000.011.54987310
TCGCACA9900.010.9593789
ATCGCAC9500.010.6839998
CCCCACT7051.0913936E-1110.4259751
CGGGCAT5401.7093043E-810.37081051
CCCACAT13400.010.1871
CGCCCAT11100.010.0905191
CGCCCCT3156.96138E-410.0004241
GCACTAT11100.09.77440757
GCCGGAT10900.09.6334371
GCGGGAT10000.09.4504011
GGCAATC14450.09.4467682
GCGAAAT13000.09.4234771
GGGCGCC3000.0052252139.3339824
CCCGTAT4956.4493906E-69.192311
ACATCGC14850.09.1923096
CGGGGAG7655.4023985E-109.1507151
CTAGCAC12250.09.14251354
Produced by FastQC (version 0.11.5)