FastQCFastQC Report
Tue 7 Jan 2020
HV3K3BGXC_n01_30_NxW_C10-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3K3BGXC_n01_30_NxW_C10-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14320874
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGC5550.016.3962438
GCGCGCA6150.015.93475139
CGCGCAC7900.011.96186940
GGGTCGC11600.011.16329647
GCCCGAT9200.011.0336971
CCGGGAT37150.011.0239681
CGCCAAT31850.010.7702931
GCGGGAT20700.010.4841261
CGGGCAT14650.010.2740521
CCCCGAT10600.010.2368591
CCGGCAT24350.010.0625941
CGGGGCT12150.09.7952051
GCCGGAT32300.09.7532841
CGGGCGT5855.9049853E-89.5735941
CGGGATA22850.09.4976552
CGCCCAT25800.09.4970611
GTCGAAT58400.09.4101741
CCCGTTT32000.09.1884061
CGGGGGT5058.307745E-69.0107911
CGGGGAG19100.08.9799431
Produced by FastQC (version 0.11.5)