FastQCFastQC Report
Tue 7 Jan 2020
HV3K3BGXC_n01_29_NxW_C10-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3K3BGXC_n01_29_NxW_C10-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8082537
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT7550.015.7629171
CGGGGAG9300.011.2912741
GCCGAAT8950.010.9506431
CGCGGAT12100.010.4141011
GGGGCCC3451.4238953E-410.14446460
GGGGAAT18000.010.1119631
GGCGCGC2800.00295751249.99985238
CGGGGAT13050.09.9242081
CGCCCAT13100.09.8863291
GCGCGAT3300.00106613869.5462591
GGGGCAT8505.456968E-129.4713861
CCGGGAT23050.09.4151111
CGGGGCT5957.667768E-89.4125581
GTCGGAT32750.09.1916141
GGGTCGC6908.294592E-99.13001747
CCGCAAT14600.09.1103561
TAGCGCC7352.5465852E-99.0472629
GCCCGCT4703.5011333E-58.9369221
CGGGACT11150.08.7899791
CGCGGGC3600.00235854428.749665
Produced by FastQC (version 0.11.5)