FastQCFastQC Report
Tue 7 Jan 2020
HV3K3BGXC_n01_28_NxW_C10-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3K3BGXC_n01_28_NxW_C10-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9095400
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT9900.012.3746581
CGCGCAT3501.3609735E-511.0008181
CGGGGAT13200.010.8720211
GCCGGAT17800.010.4221231
GGGTCGC8650.010.11524847
CGTTCGG10450.010.04765542
CGCGTAT6101.0830263E-89.7548241
GGGGAAT17600.09.7450431
GCGCGAT5201.1834018E-69.4237781
CGCGGGC3803.758812E-49.2112111
CGCGGAT14100.09.1850811
CCCGGGG7302.248271E-99.1102671
GCGCGTA5007.3351985E-69.09967570
AGACGCG10050.09.054852522
GGCGCGC3500.00182527268.99997638
CGGGAAT24950.08.9786231
CCGGCAT14450.08.96260451
CGCCAAT19500.08.7955261
CTCGGAT39250.08.7395041
GCGAAAT24000.08.6048061
Produced by FastQC (version 0.11.5)