FastQCFastQC Report
Tue 7 Jan 2020
HV3K3BGXC_n01_27_NxW_C9-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3K3BGXC_n01_27_NxW_C9-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9254884
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT13300.012.3689931
CGGGCGC2952.8955561E-511.86469915
GGGGAAT24400.011.619391
CGGGCAT9900.010.6065511
CGGCAAT20050.010.4742991
CCGGGAT20700.010.1453961
GCCGAAT12250.010.0004631
CCGGGGT10650.09.5309561
GTCGAAT29750.09.0592421
GCGGAAT19000.09.0267331
CCCGTAT11350.08.9431441
CCGGCAT17250.08.725041
CGCCCTT11300.08.6729671
GGGGTAT12600.08.3337181
CCCCACT12600.08.3337181
CGGCAGT14800.08.277411
CGGGGAG11450.08.2536561
CCGCGCT5957.3168376E-68.2356751
CCGGGTT27350.08.1905071
CCGGGCA9001.3224053E-98.1670451
Produced by FastQC (version 0.11.5)