FastQCFastQC Report
Tue 7 Jan 2020
HV3K3BGXC_n01_26_NxW_C9-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3K3BGXC_n01_26_NxW_C9-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3323427
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGC1304.3484833E-621.53875738
GCGCGCA1803.6637412E-617.49944939
CCCGGGG2201.3956997E-615.9102671
CGCGCAC1901.1352409E-414.73638140
GGGCCGA1750.001019014913.99935166
GCGGCCT2902.4283234E-512.0698581
CGGGCAT3501.0400163E-612.0008881
CGGGGAT6251.8189894E-1211.760871
CTCGCAT7850.011.5932141
CGGGTAT5904.7293724E-1111.272021
GTCCGCT2903.2459744E-410.8628721
GCCGAAT4301.0667154E-610.5821781
CCCGGAT6653.8198777E-1110.5270941
CCGACTG5057.111157E-810.3968092
CGCGCAA2700.002184868610.3711381
GCGCACC2700.00218678210.37004541
CCTCGCG2800.00295750669.99953667
CGCCCAT5351.6523518E-79.8138091
GGCCAAT9400.09.6815661
GCCGGAT8353.6379788E-129.6414321
Produced by FastQC (version 0.11.5)