FastQCFastQC Report
Tue 7 Jan 2020
HV3K3BGXC_n01_25_NxW_C9-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3K3BGXC_n01_25_NxW_C9-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9671280
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCGC4201.8189894E-1215.00004615
CGGGGGT3801.5559635E-812.8954441
CGGGCAT11350.012.6438651
CGGCAAT22900.011.7692041
CCCGTAT10900.010.5969111
GCCGAAT15200.010.5926871
CCCCCCT2650.001871133510.5666171
CCCGCAT9950.010.5533421
GGCGGGC6251.5079422E-910.08003113
GGGCGCA6351.9845174E-99.9212916
CGCGTAT6402.2700988E-99.844291
CGGGAAT23200.09.6557021
GCGGGCG5906.741902E-89.49155514
CCCCACT16100.09.3483391
TCGGGCG7151.54796E-99.3005357
GCCCGAT9353.6379788E-128.9844491
ATCGGGC8202.1100277E-108.9639056
CCCGTTT18850.08.9129551
CGCCCAT16550.08.8826621
GGCGAAT28800.08.8720141
Produced by FastQC (version 0.11.5)