FastQCFastQC Report
Fri 26 Mar 2021
HV3J5BGXG_n01_AKM228.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3J5BGXG_n01_AKM228.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16904099
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAA549020.32478513051775193No Hit
CCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAGA493400.29188186841546543No Hit
GTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCG402110.23787721546117305No Hit
CTAAGAAGTTATACGAACCCGAAGATTCGCCAACTATTTAGCAGGCTAGA382450.22624690023407934No Hit
CCCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAG378140.22369722278602366No Hit
CCCCAGTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGC377490.22331270066508724No Hit
CTGAGATCCAGCTATCCTGAGGGAAACTTCGGAAGGAACCAGCTACTAGA315940.1869014136748726No Hit
GTACGATTGAGTGCAAATTAATAGCCTCCCTTGGTGTTTTAAGGGCCGTT298100.17634776038640096No Hit
CGCGCTTGTGCTCCGAAGAACACAATCTTTGCATCTCAGTTAGGGAATAT291760.17259719077603602No Hit
GCAAGTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCA288110.17043795117385435No Hit
GCCCCAGTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTG269370.15935188264100913No Hit
CAGCTTTGCAACCATACTACCCCCGGAACCGAAAGACTTTCGTTTCCGGA252480.1493602232215985No Hit
TCCGGTTCCACTCCAGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAG237280.1403683213166227No Hit
GCTCAATCTCGTGCGGCTAAACACCGCTTATCCCTCTAAGAAGTTATACG227730.13471880400132535No Hit
CCGCGCTTGTGCTCCGAAGAACACAATCTTTGCATCTCAGTTAGGGAATA221070.13077893119296094No Hit
GTTCAGTTATAATCTCTCAAATGGTAGCTTCAGACCATTGATCTGTCGAT210920.12477447038141459No Hit
CTTTGCAACCATACTACCCCCGGAACCGAAAGACTTTCGTTTCCGGATAG196590.11629723654599988No Hit
GTCGTATGCAAGTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGC186660.11042292168307816No Hit
CGACTAACCAAGTTCGAATGCGTTACCGCGCTTGTGCTCCGAAGAACACA169460.10024787479060553No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)