FastQCFastQC Report
Fri 26 Mar 2021
HV3J5BGXG_n01_AKM227.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3J5BGXG_n01_AKM227.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16551828
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAGA592070.3577067137237047No Hit
CCCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAG547600.33083959064823537No Hit
GTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAA495670.2994654125212031No Hit
CCCCAGTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGC476360.2877990273944364No Hit
CTAAGAAGTTATACGAACCCGAAGATTCGCCAACTATTTAGCAGGCTAGA448560.2710032994542959No Hit
GTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCG407950.24646824507842882No Hit
CTGAGATCCAGCTATCCTGAGGGAAACTTCGGAAGGAACCAGCTACTAGA384840.23250604102459257No Hit
CAGCTTTGCAACCATACTACCCCCGGAACCGAAAGACTTTCGTTTCCGGA349710.21128179920670997No Hit
GCCCCAGTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTG287540.17372099323410078No Hit
TCCGGTTCCACTCCAGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAG281730.17021080692718654No Hit
CGCGCTTGTGCTCCGAAGAACACAATCTTTGCATCTCAGTTAGGGAATAT280200.16928643772760327No Hit
GTACGATTGAGTGCAAATTAATAGCCTCCCTTGGTGTTTTAAGGGCCGTT264850.1600125375879933No Hit
CTTTGCAACCATACTACCCCCGGAACCGAAAGACTTTCGTTTCCGGATAG258300.15605527075317602No Hit
GCAAGTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCA249050.15046676415438826No Hit
GTTCAGTTATAATCTCTCAAATGGTAGCTTCAGACCATTGATCTGTCGAT204890.12378693157033772No Hit
GCTCAATCTCGTGCGGCTAAACACCGCTTATCCCTCTAAGAAGTTATACG202900.12258464744800393No Hit
CTTCAAAGTCCTTTGCAACTTTCCCTCACGGTACTTGTTCGCTATCGCAA197380.1192496683749976No Hit
GTCAATTTCTTTAAGTTTCAGCTTTGCAACCATACTACCCCCGGAACCGA197200.11914091905739958No Hit
CCGCGCTTGTGCTCCGAAGAACACAATCTTTGCATCTCAGTTAGGGAATA191790.11587239790070317No Hit
CCGGAGTCGAACCCTTATTCTCCGTTACCCGTAACAACCATGGTAGTCCA189150.11427740790926537No Hit
GCCCGTTCCCTTGGCTGTGGTTTCGCTAGAAAGTAGTTAGGGACAGTAGG182280.1101268089542738No Hit
CTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAGAACCGAAGTCCT180130.10882785877185289No Hit
AAGCTCTCGTGTTGCCACGAGATAGAGATGCCTCCCGACACCGGGAAGCA177530.10725703529543686No Hit
CTCAGGCATAGTTCACCATCTTTCGGGTCGTTTGCCAACTGCTCAACCTC177060.10697307874393089No Hit
CGACTAACCAAGTTCGAATGCGTTACCGCGCTTGTGCTCCGAAGAACACA171220.10344476755075027No Hit
CCGGTTCCACTCCAGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAGT171170.10341455940697304No Hit
CGGTTCCACTCCAGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAGTC170940.1032756019455978No Hit
CTCTCGTGTTGCCACGAGATAGAGATGCCTCCCGACACCGGGAAGCAAGA169250.10225456668592739No Hit
GTCGTATGCAAGTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGC167140.10097978301852822No Hit
CTGGAAACTTACACCTATACGCCACATTTCGAAGCAGCCAAAGACTGATC167000.10089520021595198No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)