FastQCFastQC Report
Fri 26 Mar 2021
HV3J5BGXG_n01_AKM226.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3J5BGXG_n01_AKM226.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15572766
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAA447160.2871423098504145No Hit
CCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAGA435220.2794750784799566No Hit
GTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCG360450.2314617711458581No Hit
CTAAGAAGTTATACGAACCCGAAGATTCGCCAACTATTTAGCAGGCTAGA337900.21698136349059632No Hit
CCCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAG330680.2123450644541888No Hit
CCCCAGTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGC302940.19453191552483354No Hit
CTGAGATCCAGCTATCCTGAGGGAAACTTCGGAAGGAACCAGCTACTAGA271610.17441346001089336No Hit
CGCGCTTGTGCTCCGAAGAACACAATCTTTGCATCTCAGTTAGGGAATAT251900.16175674892950936No Hit
CAGCTTTGCAACCATACTACCCCCGGAACCGAAAGACTTTCGTTTCCGGA247390.15886066739845703No Hit
GCAAGTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCA229280.1472313910065816No Hit
GTACGATTGAGTGCAAATTAATAGCCTCCCTTGGTGTTTTAAGGGCCGTT227540.14611405578174103No Hit
GCCCCAGTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTG216360.13893485588879972No Hit
TCCGGTTCCACTCCAGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAG209360.1344398291221996No Hit
GCTCAATCTCGTGCGGCTAAACACCGCTTATCCCTCTAAGAAGTTATACG203470.1306575851714461No Hit
CTTTGCAACCATACTACCCCCGGAACCGAAAGACTTTCGTTTCCGGATAG191090.12270780926137334No Hit
CCGCGCTTGTGCTCCGAAGAACACAATCTTTGCATCTCAGTTAGGGAATA175350.11260042050333255No Hit
GTTCAGTTATAATCTCTCAAATGGTAGCTTCAGACCATTGATCTGTCGAT171280.10998688351189508No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)