FastQCFastQC Report
Fri 26 Mar 2021
HV3J5BGXG_n01_AKM225.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3J5BGXG_n01_AKM225.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16295771
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGCCATAA495660.3041648044759588No Hit
CCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAGA480030.2945733589407951No Hit
CCCGGTTTGTCCCTCTTAACCATTAAATCAGTTCTAGAACCAACAAAAAG361470.22181828647444787No Hit
GTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCAAGCG358950.22027187299085144No Hit
CTAAGAAGTTATACGAACCCGAAGATTCGCCAACTATTTAGCAGGCTAGA340330.20884559558427768No Hit
CCCCAGTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGC338530.20774101452456592No Hit
CTGAGATCCAGCTATCCTGAGGGAAACTTCGGAAGGAACCAGCTACTAGA261280.1603360773786033No Hit
CGCGCTTGTGCTCCGAAGAACACAATCTTTGCATCTCAGTTAGGGAATAT252860.15516909264372947No Hit
GCCCCAGTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTG246770.15143192672503805No Hit
GCAAGTGATTTAGCGCCCCGACATTGAACCAGAGTGATCTGGCGTTGGCA244580.15008801976905542No Hit
CAGCTTTGCAACCATACTACCCCCGGAACCGAAAGACTTTCGTTTCCGGA236730.1452708190364236No Hit
TCCGGTTCCACTCCAGCGATTTCACGTTCTCTTGAACTCTCTCTTCAAAG235070.14425214983691168No Hit
GTACGATTGAGTGCAAATTAATAGCCTCCCTTGGTGTTTTAAGGGCCGTT232340.14257686856301552No Hit
GCTCAATCTCGTGCGGCTAAACACCGCTTATCCCTCTAAGAAGTTATACG204000.1251858534339983No Hit
CTTTGCAACCATACTACCCCCGGAACCGAAAGACTTTCGTTTCCGGATAG183580.11265499496771278No Hit
CCGCGCTTGTGCTCCGAAGAACACAATCTTTGCATCTCAGTTAGGGAATA183130.11237884970278485No Hit
GTTCAGTTATAATCTCTCAAATGGTAGCTTCAGACCATTGATCTGTCGAT180190.11057470063858899No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)