FastQCFastQC Report
Wed 7 Aug 2019
HV3HYBGX7_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3HYBGX7_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27780831
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8090942.9124182786324857No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGACGG15350.033.74798670
GCGATCT139350.022.50585770
AGCGATG14750.021.59453670
CGAGCGG23850.020.98660570
CGATCTA92900.020.49640770
CTACGGT7700.019.09313
CCTACGG8200.018.78278412
GCGATCG7100.018.73353670
AGCGATC183000.017.90244169
TACGGTG8500.017.7070914
GTAGATC22850.017.00322570
CCGTATC4750.016.211461
CGTAATC10450.015.74091422
CGAATTG9800.015.35644622
CCGACGC21750.015.12735970
ACGGTGA10450.015.07374915
CGCACCT10600.014.5298438
CTAGACG11150.014.438523
GAGCGAT238100.014.14174969
CGTCTAG11650.014.1195321
Produced by FastQC (version 0.11.5)