FastQCFastQC Report
Wed 7 Aug 2019
HV3HYBGX7_n02_ba175.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3HYBGX7_n02_ba175.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42891312
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG43000.025.884711
CGATCTA595550.022.40080370
CTACGGT42550.021.88608413
CGACGAC25250.021.62181570
GCGATCT869150.021.39311670
TACGGTG45300.020.71125414
AGCGATA24950.020.33885270
CTAGACG50050.019.5822893
TTGACGG53250.019.12724731
GTAGATC111350.018.98347370
CCTACGG49800.018.34865612
GACGCAC53000.018.098246
ACGCACC53400.017.5039777
TAGACGC90000.017.4638564
ATCATCG44050.017.4009430
TCCGCAA51400.017.36447733
TGCGACC45850.017.10398110
AACTGCG50850.017.0736927
CGTACGT39050.016.94048934
ACGGTGA56050.016.86328715
Produced by FastQC (version 0.11.5)