FastQCFastQC Report
Wed 7 Aug 2019
HV3HYBGX7_n02_ba174.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3HYBGX7_n02_ba174.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38198496
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGATA28050.027.19865470
CGTCTAG37000.024.3117711
CGATCTA528350.022.71936670
GCGATCT785900.022.5368370
CGACGAC25300.021.44050270
GTAGATC107400.019.71400570
TTGACGG42950.019.15075731
CTAGACG37050.018.422943
CTACGGT33400.018.34315713
AGCGATC1063600.017.92596670
GACGCAC37250.017.5740666
TACGGTG35850.017.28423314
TAGACGC68900.016.6641244
TGACGGC50000.016.6606832
TGCGACC36200.015.86057610
TAGGACG214700.015.4399484
GATCTAG423900.015.37234270
GACGGCT52400.015.36320433
CCTACGG40800.015.18806612
ACTGCGA63950.015.054658
Produced by FastQC (version 0.11.5)