FastQCFastQC Report
Wed 7 Aug 2019
HV3HYBGX7_n02_ba173.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3HYBGX7_n02_ba173.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36864050
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAC34300.036.42445470
AGCGATA25900.033.64484470
CGTCTAG40150.026.2398051
CGATCTA362600.025.13229270
GCGATCT559300.023.98977970
GCCGACC41250.023.16101670
CTACGGT36550.021.55128713
TTGACGG49150.021.15036631
TACGGTG37950.021.12435714
TGACGGC54800.018.84217832
CTAGACG45250.018.642473
TCCGCAA42350.018.59591533
CCTACGG43200.018.31514512
AGCGATC759650.018.1694770
TGCGACC43650.017.88554610
GACGGCT55800.017.87718633
CGTCTTA39000.017.59290915
TAGACGC81650.017.5344734
GACGCAC45800.017.5036036
GTAGATC77600.017.40782470
Produced by FastQC (version 0.11.5)