FastQCFastQC Report
Wed 7 Aug 2019
HV3HYBGX7_n02_ba172.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3HYBGX7_n02_ba172.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31578020
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGATA22100.036.10489370
CGACGAC26800.035.1269970
GCGATCT511800.024.37713670
CGATCTA329500.024.24788970
GTAGATC67300.021.63225270
CGTCTAG32600.020.9362531
GCCGACC34700.019.96913170
TACGGTG28900.019.01833714
CTACGGT28350.018.77070213
AGCGATC701300.018.7133270
CGCCGTA6750.017.62745759
AACTGCG42350.017.3592557
TGCGACC37750.017.34237110
ACGAGCG1541850.016.99828770
GCGACCA38850.016.94137211
CGACCAA40300.016.67980612
GACGCAC35350.016.6371756
ATCATCG39700.016.57457530
TTGACGG39050.016.49215531
CCTACGG33000.016.33810212
Produced by FastQC (version 0.11.5)