FastQCFastQC Report
Wed 7 Aug 2019
HV3HYBGX7_n02_ba171.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3HYBGX7_n02_ba171.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30693941
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGATA20200.031.53128870
CGACGAC27350.030.70970270
CGTCTAG33650.024.7556151
GTAGATC71650.024.03093170
GCGATCT535950.023.94464170
CGATCTA351750.023.56962470
CTACGGT28950.021.76774213
TACGGTG29900.020.72421614
GCCGACC28850.020.25780370
TGCGACC36800.019.59761210
CGACCAA38050.019.23042512
AACTGCG40350.019.174287
AGCGATC736500.019.02105170
GACGCAC35100.018.5511656
TTGACGG40850.018.50719831
GCGACCA38500.018.45928411
GCGATCA12900.018.44764370
CCTACGG33650.018.41558312
ATCATCG40600.017.931430
ACGCACC36000.017.5041227
Produced by FastQC (version 0.11.5)