FastQCFastQC Report
Wed 7 Aug 2019
HV3HYBGX7_n02_ba170.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3HYBGX7_n02_ba170.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18136346
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAC25050.047.08169670
GCCGACC22850.046.40730370
AGCGATA9000.041.6074670
CGTCTAG23400.032.757961
CGATCTA93550.030.82572770
GCGATCT162100.029.36201370
CTACGGT17050.027.92491113
GACGCAC18200.027.6973636
TACGGTG17400.027.56341614
ACGCACC18250.026.8551797
CCTACGG18700.026.0230912
TTGACGG29100.025.01809131
ACGGTGA19850.024.33700415
TCTGACC59250.024.21728370
GCGATCA5850.023.33128570
CTAGACG21700.023.0668183
TCCGCAA20950.022.7216533
TGACGGC32250.022.57458732
AGCGATC239300.022.53667470
GTCCGCA21300.022.51267832
Produced by FastQC (version 0.11.5)