FastQCFastQC Report
Wed 7 Aug 2019
HV3HYBGX7_n02_ba169.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3HYBGX7_n02_ba169.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34245347
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA20250.055.4754359
TCGCCGT21450.051.88192458
CGTATCA22350.050.57665662
CCGTATC24800.045.156361
GTGGTCG25550.043.8319954
GATCTCG29100.039.9272147
GTCGCCG28500.039.1708857
TCGGTGG28950.038.56358351
CGGTGGT29300.038.1029852
AGCGATA20350.037.4905970
TCTCGGT31050.036.96956649
ATCTCGG31600.036.54750448
GGTCGCC31850.034.8316256
CTCGGTG34450.033.1178250
CGACGAC21150.031.2738770
CGTCTAG44900.027.4395281
CTACGGT37550.026.1056413
TACGGTG38400.025.8003914
GCGATCT579600.025.64993770
GCCGACC22700.024.97579870
Produced by FastQC (version 0.11.5)