FastQCFastQC Report
Wed 7 Aug 2019
HV3HYBGX7_n02_ba168.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3HYBGX7_n02_ba168.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38958998
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGATA23850.028.76075770
CGTCTAG45250.027.92311
GCGATCT618550.023.5992470
CGATCTA406200.023.45205170
CGACGAC25650.023.0585570
CTACGGT40250.022.87510313
CGCCGTA9950.022.5098359
TACGGTG40450.022.50183714
TTGACGG55700.021.80488231
GACGCAC46900.020.377026
CTAGACG48750.020.1043073
CCTACGG47000.019.5902512
TGACGGC62600.019.4015732
GTAGATC78700.019.2995770
TGCGACC47600.019.04880110
ACGGTGA49800.018.6279815
ATCATCG48150.018.60879130
AACTGCG52000.018.5133617
GACGGCT63250.018.3167633
ACGCACC51550.018.1316997
Produced by FastQC (version 0.11.5)