FastQCFastQC Report
Wed 7 Aug 2019
HV3HYBGX7_n02_ba167.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3HYBGX7_n02_ba167.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26872786
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAC26300.035.26315770
AGCGATA15150.028.18241370
CGTCTAG33550.028.1686021
GCCGACC28350.026.54094770
CGATCTA217550.025.12771270
GCGATCT335050.024.48380970
TTGACGG40700.022.27323331
CTACGGT27650.021.77751213
GCGATCA10050.021.2419770
TACGGTG29150.020.65615714
AACTGCG33150.020.1699757
TGACGGC45500.019.69286332
GACGCAC33050.019.3833456
GACGGCT45150.019.22538833
ACGCACC32850.018.9688157
AGCGATC460800.018.8807770
TCCGCAA32100.018.64542233
CTAGACG34050.018.5042423
CCTACGG33400.018.34297412
TGCGACC31950.018.18907210
Produced by FastQC (version 0.11.5)