FastQCFastQC Report
Wed 7 Aug 2019
HV3HYBGX7_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3HYBGX7_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27780831
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11102223.996359936101263No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG8950.028.5901451
TACGGTG9350.021.70547714
CTACGGT9400.021.58998313
CTAGACG10900.020.5464553
TCCGCAA11050.018.05276533
CCTACGG11450.018.0300912
ACGGTGA11350.017.88069215
GCTACGC32400.017.4996570
GACGGCT12300.017.35624933
GCTCGAA9550.016.85414311
ACGCACC12900.016.5457947
CGCTACG38550.016.16050370
CGCACCT13400.015.9282998
TTGGACG10000.015.74591217
CGTAATC10000.015.74585522
TAAGGCG16500.015.69665370
ATTCGAA11800.014.82705932
TAGGGCG13350.014.67855131
TGCTCGA10850.014.51212710
TGTCGAA10650.014.45623719
Produced by FastQC (version 0.11.5)