FastQCFastQC Report
Wed 7 Aug 2019
HV3HYBGX7_n01_ba175.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3HYBGX7_n01_ba175.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42891312
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA57200.027.53466270
CGTCTAG43550.027.2422471
CTACGGT45850.025.26159713
TACGGTG48850.023.71019214
GACGCAC51650.023.034986
CTAGACG53000.022.7120173
ATCTCGT86850.022.5268169
GCTACGC621950.022.3632970
ACGCACC53500.021.8456947
CGCTACG890150.021.6646570
CCTACGG54850.021.49945312
TATCTCG93450.020.9734368
TCCGCAA54450.020.95406733
TTGACGG55700.020.2931931
ACGGTGA60600.019.45942115
GTCCGCA59250.019.43103432
TAGACGC95100.019.280754
AACTGCG53450.018.5927587
TGCGACC46950.018.11084710
TGACGGC64050.017.8109432
Produced by FastQC (version 0.11.5)