FastQCFastQC Report
Wed 7 Aug 2019
HV3HYBGX7_n01_ba174.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3HYBGX7_n01_ba174.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38198496
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG33650.023.2968441
CTACGGT37950.022.77509513
TACGGTG38750.022.48541614
CTAGACG43900.020.8836633
GACGCAC42950.020.4494486
CCTACGG43850.019.87023712
ATCTCGT48550.019.39223569
TCCGCAA44000.019.32831433
ACGCACC45300.019.3111327
TAGACGC75450.019.0612474
ATCGCGT19550.018.6187969
GTCCGCA46350.018.1950232
ACGGTGA50850.017.68537915
GATCTCG59550.017.63225268
TCTCGTA31200.017.27558170
TTGACGG41550.017.26589631
GGTACGA34750.016.81649618
CGTGATT89550.016.68623528
TCGCGTA9050.016.62977870
CCAGAGA1131250.016.49983270
Produced by FastQC (version 0.11.5)