FastQCFastQC Report
Wed 7 Aug 2019
HV3HYBGX7_n01_ba173.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3HYBGX7_n01_ba173.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36864050
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG40800.029.5923731
TCTCGTA43000.025.39493470
TTGACGG51800.022.69965231
CTACGGT40750.022.5845113
TACGGTG42650.021.90653214
ATCTCGT53400.021.3664669
GACGCAC45700.020.980816
CTAGACG46200.020.9049473
TCCGCAA45450.020.17494833
CCTACGG46100.019.96358712
ACGCACC47000.019.5813587
TGACGGC60250.019.341332
TAGACGC83600.019.0034894
GTCCGCA49600.018.76715532
GGTACGA41250.018.57841318
GACGGCT61100.018.50147633
ACGGTGA51950.018.05222115
AACTGCG46650.018.0029857
GCTCGAA42650.017.96835111
GTACGAG43650.017.63704119
Produced by FastQC (version 0.11.5)