FastQCFastQC Report
Wed 7 Aug 2019
HV3HYBGX7_n01_ba172.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3HYBGX7_n01_ba172.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31578020
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA38550.031.14063370
CGTCTAG35400.029.7566341
ATCTCGT48400.024.36929169
CTACGGT33000.023.32830613
CTAGACG35950.022.5820263
TACGGTG35800.021.5037814
ACGCACC37750.021.134557
TTGACGG47000.021.07136231
GACGCAC38450.020.9319366
TGCGACC41000.020.65382410
CGACCAA40450.020.50234812
AACTGCG43200.020.4933017
GATCTCG57250.020.41895368
CCTACGG37900.020.12755612
ATCATCG42400.019.39566430
TCCGCAA39250.019.34927433
TAGGCAT430250.019.34410770
CTAGGCA673100.019.33767770
GCGACCA43850.019.07228711
TGACGGC53900.018.37324532
Produced by FastQC (version 0.11.5)