FastQCFastQC Report
Wed 7 Aug 2019
HV3HYBGX7_n01_ba171.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3HYBGX7_n01_ba171.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30693941
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA44750.034.2566870
CGTCTAG38350.030.1135921
ATCTCGT59300.027.50379469
CTACGGT33250.027.04701613
AGACGGC41400.026.9683670
TACGGTG35450.025.36845814
TATCTCG68400.024.25409168
CGGACTC548950.024.02383470
ACGCACC37800.023.7906977
CTAGACG38800.023.5382753
CCTACGG38650.023.35855512
GACGCAC39100.023.2683336
TTGACGG46650.023.17997731
TCCGCAA40850.021.84685733
ACGGTGA43300.021.5774715
GTCCGCA41500.021.33347532
TGACGGC52750.020.63132532
CGCACCT44400.020.490648
GACGGCT52050.019.9699333
TGCGACC37300.019.60670910
Produced by FastQC (version 0.11.5)