FastQCFastQC Report
Wed 7 Aug 2019
HV3HYBGX7_n01_ba170.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3HYBGX7_n01_ba170.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18136346
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG24500.036.994861
TACGGTG20950.029.56540314
CTACGGT21850.028.66791713
ACGCACC22000.028.1537847
GACGCAC22350.027.8698526
CCTACGG23350.026.82644312
TTGACGG32250.026.69396631
ACGGTGA23750.026.22728315
CTAGACG23900.025.6229953
TGACGGC34300.024.99611532
CGCACCT25050.024.5861158
GACGGCT34700.024.20606633
TCCGCAA25350.024.16031333
CTCCTGA201300.024.0283970
GTCCGCA26300.023.28535532
AACTGCG25800.022.9220357
TGCGACC26400.021.20806310
GCGACCA27200.020.58475111
CGACCAA26850.020.46219812
CTGCGAC29650.019.9455959
Produced by FastQC (version 0.11.5)