FastQCFastQC Report
Wed 7 Aug 2019
HV3HYBGX7_n01_ba169.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3HYBGX7_n01_ba169.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34245347
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG24650.043.32172849
TGCCGTC25700.040.59515851
CCGTCTT27150.038.42759753
GCCGTCT28000.036.7601552
CGTCTAG49450.033.1216131
CTCGTAT29500.030.7375745
GACGCAC44850.028.711836
TCTCGTA51200.028.3691470
CTACGGT44700.028.18162313
TACGGTG45650.027.51845714
ACGCACC46700.027.2742237
CTAGACG48800.026.530883
CCTACGG47600.026.4648712
TTGACGG61100.025.83088931
ATCTCGT61650.025.49034169
TCCGCAA50300.024.90937233
ACGGTGA51400.024.57625615
CGCACCT51950.024.382968
TGACGGC66250.023.92763932
GTCCGCA53000.023.63712732
Produced by FastQC (version 0.11.5)