FastQCFastQC Report
Wed 7 Aug 2019
HV3HYBGX7_n01_ba168.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3HYBGX7_n01_ba168.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38958998
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA47150.030.43519670
CGTCTAG46050.028.7273031
CTACGGT46500.023.25315513
ATCTCGT62550.023.10964869
TACGGTG48350.022.5080914
CTAGACG50400.022.0090713
GACGCAC50950.021.840396
TTGACGG57600.021.8107831
ACGCACC50200.021.608797
TCCGCAA51650.021.20888333
CCTACGG53100.020.5606312
ACCGTAA16400.019.42099270
TGACGGC65200.019.37531732
ACGGTGA56950.019.29353115
GTCCGCA56500.019.2619932
CCGTACT786900.019.24603570
CGTACTA520550.019.07527470
GACGGCT66150.019.04655533
TGCGACC47300.018.93860210
GATCTCG76450.018.77061868
Produced by FastQC (version 0.11.5)