FastQCFastQC Report
Wed 7 Aug 2019
HV3HYBGX7_n01_ba167.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3HYBGX7_n01_ba167.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26872786
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTAG32450.032.3532331
TAAGGCG223100.024.5833170
TTGACGG41450.023.30208831
CTACGGT29050.022.64582313
CTAGACG30350.022.1372133
TGACGGC44950.021.565132
TACGGTG31550.020.85134314
ACGCACC32900.020.5274287
GACGCAC32400.020.5206286
GACGGCT47000.020.40351733
TCTCGTA20850.020.31188670
ATCTCGT27100.020.27677569
TCCGCAA33600.020.10344133
CTAAGGC435750.019.88768270
CCTACGG33950.019.48042512
ACGGTGA36050.018.63684315
CGTCTTA30650.018.3811115
TAGACGC65300.018.1662854
GTCCGCA37200.017.87360432
AAGGCGA148700.017.79433470
Produced by FastQC (version 0.11.5)