FastQCFastQC Report
Mon 7 Oct 2019
HV3CVAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3CVAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12191054
Sequences flagged as poor quality0
Sequence length151
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG243072219.93857134912207No Hit
GGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG144148511.82412119575551No Hit
GGGGGGGGGGGGCGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7208715.913114649479857No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC4141753.3973682669275354No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT3983183.26729747895465No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC3679293.0180245284780134No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC3379342.771983456065407No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG3254992.6699824313796No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA2876182.359254581269183No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA2250851.8463128782794336No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT2105741.7272829732359483No Hit
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG2097701.720687973328639No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG1719951.410829613255753No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT1411221.157586538456806No Hit
TAAGTAGAGTCTNGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT1101950.9039005159028908No Hit
ACGATCGATAGGNAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC1019650.836391996951207No Hit
TCGATCGTTACCNTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC822520.6746914581790877No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG689600.5656606885672067No Hit
GCGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG672350.5515109686168235No Hit
TCGATCGTTACCTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC636710.5222764167889011No Hit
TAAGTAGAGTCTAGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT547700.4492638618449233No Hit
GGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG537760.44111034205902133No Hit
GCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG520840.427231312403341No Hit
ACGATCGATAGGAAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC512120.42007852643421967No Hit
GCGGGGGGGGGGCGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG483020.3962085640831383No Hit
CGATCGATACAGCTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC436930.35840215292295485No Hit
ATACACGATCTCATGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG366140.30033498334106307No Hit
GATCGATAACGCTTTTGTTGTGGAAAGGACGAAACACCGAACCCCTACCA359300.29472431177812847No Hit
GATAGGTCGCATGTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG309010.2534727514126342No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG303680.24910069301637086No Hit
TAACAATGGTCTAGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT292800.24017611602737549No Hit
ATCGATTCCTTGCTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA244030.2001713715647556No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAAACACCGAACCCCTACCAAC234660.19248540774243145No Hit
ATACTGTATCTCATGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG225240.18475843023909172No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGGGGGGGGG202130.16580190687367966No Hit
GATCGATAACGCTTTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA198510.16283251636814994No Hit
GGGGGGCGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG184060.15097956255464048No Hit
ACGATCGATAGGAAAGCTCTTGTGGAAAGGACGAAACACCGAACCCCTAC182710.14987219316721917No Hit
TAAGTAGAGTCTAGTGCAAAGGACGAAACACCGAACCCCTACCAACTGGT164400.13485298317930508No Hit
CGATCATGATCGACTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT156270.12818415864616792No Hit
GATCGATAACGCATTTGTTGTGGAAAGGACGAAACACCGAACCCCTACCA154050.12636315120907513No Hit
CGATCGATACAGCTATACTTGTGGAAAGGACGAAACACCGAACCCCTACC151510.12427965621348244No Hit
ATACACTATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG149440.12258168981943644No Hit
ATACAGTATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG144420.11846391624546983No Hit
GATAGGTCGCATCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTG142770.11711046477195491No Hit
ATACACGATCTCATGTCGAAAGGACGAAACACCGAACCCCTACCAACTGG136580.11203297106222317No Hit
GATCGCGCGGTTGTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG122210.10024563913833866No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGCA360450.0150.876487
TCGCATC212550.0150.366357
GATCTCT398100.0149.717127
AGTCTTG491400.0149.598228
ACGCATT364650.0149.267979
TCCTTGG282000.0148.546977
TCTCTTG691950.0147.93599
CGCATCT221750.0147.843498
GATCGTC176800.0147.117438
ATACAGG450900.0146.645037
TGATCGT175250.0146.089377
CCTTGGT287300.0145.727528
AACGCAT374700.0145.632898
TACAGGT450450.0145.630778
GTCTAGT158650.0144.929619
TAGGTAA513650.0144.572929
GTTACCA439750.0144.21927
TATCTCT292550.0143.716287
TCGTTAC587950.0143.612155
TACACGA502400.0143.227812
Produced by FastQC (version 0.11.5)