FastQCFastQC Report
Mon 7 Oct 2019
HV3CVAFXY_n01_PCR2_R6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3CVAFXY_n01_PCR2_R6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2267935
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG42947018.936609735287828No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT42398018.694539305579745No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG35908515.833125728911984No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC27192811.990114355129226No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC1845398.136873411275014No Hit
GATAGGTCGCATGTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG755713.3321501718523683No Hit
TAACAATGGTCTAGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT638912.8171442303240615No Hit
ATACTGTATCTCATGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG521662.300154104945688No Hit
ACGATCGATAGGAAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC353981.560803109436558No Hit
GATAGGTCGCATCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTG293041.2921005231631417No Hit
CGATCGATACAGCTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC233971.0316433231111122No Hit
ATACTGTATCTCATGTCGAAAGGACGAAACACCGAACCCCTACCAACTGG198760.8763919600870396No Hit
TAACAATGGTCTAGTGCAAAGGACGAAACACCGAACCCCTACCAACTGGT195950.864001834267737No Hit
ACGATCGATAGGAAAGCTCTTGTGGAAAGGACGAAACACCGAACCCCTAC133360.5880239072107446No Hit
CGATCGATACAGCTATACTTGTGGAAAGGACGAAACACCGAACCCCTACC82470.3636347602554747No Hit
ATACTGTATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCAACTGG59930.26424919585437856No Hit
GATAGGTCGCATGTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTG42300.18651328190622746No Hit
TAACAATGGTCTTGTGCAAAGGACGAAACACCGAACCCCTACCAACTGGT39950.17615143291143706No Hit
ACGATCGATAGGTAAGCTCTTGTGGAAAGGACGAAACACCGAACCCCTAC37910.16715646612447008No Hit
ACGATCCATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC28970.1277373469698206No Hit
CGATCGATACAGGTATACTTGTGGAAAGGACGAAACACCGAACCCCTACC28010.12350442142301256No Hit
ATACAGTATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG25120.11076155180814265No Hit
TTACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT24260.10696955600579383No Hit
ATACTGTATCTCTTGTGGAAAGGTCGAAACACCGAACCCCTACCAACTGG22690.1000469590177849No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTGT202.1207325E-6145.143469
TACAAGT151.2253725E-4145.143468
TCGTATC351.0913936E-11145.140267
GTTTGGT100.007085456145.041031
ATAAAGA151.2288238E-4145.041031
CACTATC850.0145.015444
GTACCTC202.1300675E-6145.015436
GTCGCGT100.007089195145.015436
CATTGGT351.0913936E-11145.015434
AATGGTT306.511982E-10145.015435
TAATGGT450.0145.015434
ATACTGT542150.0145.014281
ACATTGG351.0913936E-11145.009053
GCGATAT100.007092934144.98985145
TAACAAT550250.0144.961941
ACGATCG337400.0144.933551
TACTGTA542600.0144.878622
CGCATCT424050.0144.869648
TCGCATC421700.0144.864917
CGCATGT88400.0144.81518
Produced by FastQC (version 0.11.5)