FastQCFastQC Report
Mon 7 Oct 2019
HV3CVAFXY_n01_PCR2_R5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3CVAFXY_n01_PCR2_R5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2319206
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG47718520.57536070534485No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT43434318.72809056202856No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG37393716.123492264162824No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC24043310.367039409177107No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC1890118.149815066018284No Hit
GATAGGTCGCATGTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG794323.4249652682857836No Hit
TAACAATGGTCTAGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT661942.8541664690415596No Hit
ATACTGTATCTCATGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG573112.4711474530507425No Hit
ACGATCGATAGGAAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC313621.3522731486551862No Hit
GATAGGTCGCATCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTG306541.321745459437411No Hit
CGATCGATACAGCTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC240641.0375964877634847No Hit
ATACTGTATCTCATGTCGAAAGGACGAAACACCGAACCCCTACCAACTGG216600.9339403226793999No Hit
TAACAATGGTCTAGTGCAAAGGACGAAACACCGAACCCCTACCAACTGGT202140.8715913980905534No Hit
ACGATCGATAGGAAAGCTCTTGTGGAAAGGACGAAACACCGAACCCCTAC116500.5023270895297787No Hit
CGATCGATACAGCTATACTTGTGGAAAGGACGAAACACCGAACCCCTACC85050.3667203344592934No Hit
ATACTGTATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCAACTGG65160.28095822449579727No Hit
GATAGGTCGCATGTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTG41870.18053592479495137No Hit
TAACAATGGTCTTGTGCAAAGGACGAAACACCGAACCCCTACCAACTGGT41020.17687087736061394No Hit
ACGATCGATAGGTAAGCTCTTGTGGAAAGGACGAAACACCGAACCCCTAC33460.14427351429756563No Hit
CGATCGATACAGGTATACTTGTGGAAAGGACGAAACACCGAACCCCTACC28650.12353365763972671No Hit
TTACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT25580.11029636867100207No Hit
ACGATCCATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC24410.10525153867314933No Hit
ATACTGTATCTCTTGTGGAAAGGTCGAAACACCGAACCCCTACCAACTGG24410.10525153867314933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACT351.0913936E-11145.124017
TAGGTTA151.226031E-4145.124018
TCTTTGT151.226031E-4145.124019
ATACGGG151.226031E-4145.124017
CCCAAGT202.1221585E-6145.1248
TATCTCC202.1221585E-6145.1247
GTTTGGT202.1285487E-6145.036361
ATACTTA100.007086147145.036361
ATACTGA2650.0145.036361
TAACATG100.007086147145.036361
TATTGTA306.493792E-10145.026982
AAAGTAT151.2293017E-4145.026983
TACTGAA2400.0145.026982
ACCGTAT450.0145.026983
ATAGTTC700.0145.026982
CCGTATC450.0145.023864
AATGGTT253.709465E-8145.023855
CTTATCT100.007087975145.023854
ATGGTTT253.709465E-8145.023856
CAATGTG100.007087975145.023854
Produced by FastQC (version 0.11.5)