FastQCFastQC Report
Mon 7 Oct 2019
HV3CVAFXY_n01_PCR2_R4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3CVAFXY_n01_PCR2_R4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2225974
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG48574621.82172837598283No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT39114117.57167873479205No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG35588915.988012438599911No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC23013210.338485534871475No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC1826788.206654704861782No Hit
GATAGGTCGCATGTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG748263.361494788348831No Hit
TAACAATGGTCTAGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT595542.6754131000631634No Hit
ATACTGTATCTCATGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG588562.6440560401873516No Hit
ACGATCGATAGGAAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC300461.3497911476054976No Hit
GATAGGTCGCATCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTG295781.328766643276157No Hit
CGATCGATACAGCTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC229041.0289428358103014No Hit
ATACTGTATCTCATGTCGAAAGGACGAAACACCGAACCCCTACCAACTGG228571.0268314005464574No Hit
TAACAATGGTCTAGTGCAAAGGACGAAACACCGAACCCCTACCAACTGGT181710.8163168123257505No Hit
ACGATCGATAGGAAAGCTCTTGTGGAAAGGACGAAACACCGAACCCCTAC113410.5094848367501148No Hit
CGATCGATACAGCTATACTTGTGGAAAGGACGAAACACCGAACCCCTACC81960.3681983706907628No Hit
ATACTGTATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCAACTGG67020.30108168379325184No Hit
GATAGGTCGCATGTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTG39100.17565344429000518No Hit
TAACAATGGTCTTGTGCAAAGGACGAAACACCGAACCCCTACCAACTGGT36670.16473687473438592No Hit
ACGATCGATAGGTAAGCTCTTGTGGAAAGGACGAAACACCGAACCCCTAC32550.1462281230598381No Hit
CGATCGATACAGGTATACTTGTGGAAAGGACGAAACACCGAACCCCTACC28540.1282135370853388No Hit
ATACTGTATCTCTTGTGGAAAGGTCGAAACACCGAACCCCTACCAACTGG25680.11536522888407502No Hit
ACGATCCATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC23770.10678471536504919No Hit
ATACTGTATCTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTGG22580.10143874097361424No Hit
TTACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT22460.10089965111901578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACT351.0913936E-11145.131157
GTTACCT100.0070722993145.131157
TACCTTC100.0070722993145.131159
ATAAGTA351.0913936E-11145.131158
TCGTATC253.6929123E-8145.131157
TTACCTT100.0070722993145.131158
TTACCAT306.475602E-10145.131158
TACGTAA400.0145.131159
CGCATGT89400.0145.049998
GTTTGGT100.0070879995145.023541
ACTATCG450.0145.023541
TAAAATG151.229411E-4145.023541
ATACTCG100.0070879995145.023541
ACGATTG202.1294654E-6145.023541
CGCTCGA2850.0145.023541
TTAACAA100.0070879995145.023541
ACGACCG100.0070879995145.023541
ATATTGT450.0145.023541
AAGTAGA500.0145.010512
TACTATA750.0145.010512
Produced by FastQC (version 0.11.5)