FastQCFastQC Report
Mon 7 Oct 2019
HV3CVAFXY_n01_PCR2_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3CVAFXY_n01_PCR2_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59620179
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACTGTATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG50451248.462108106049127No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA48204848.085322923971765No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT47429187.955222677207997No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG41041946.8839008349840745No Hit
GATAGGTCGCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG39722006.6625093493932654No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC32073365.379614844832989No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC31892615.349297928139397No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT30164745.059484977393308No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT29414974.9337272201078095No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC28236374.736042473136486No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA22452783.765969907604605No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG18390133.0845479346849998No Hit
GATAGGTCGCATGTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG8352321.4009216577494676No Hit
TAACAATGGTCTAGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT7180971.204452942014817No Hit
TCGATCGTTACCTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC6988071.172098124697009No Hit
ATACTGTATCTCATGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG6073411.0186836238784187No Hit
ATCGATTCCTTGCTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA5966641.000775257652279No Hit
ATACACGATCTCATGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG4999750.8386003000762544No Hit
TAAGTAGAGTCTAGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT4501420.7550161833630187No Hit
ACGATCGATAGGAAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC4158260.6974584896835013No Hit
GATCGCGCGGTTGTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG3977090.6670711270424062No Hit
CGATCATGATCGACTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT3614370.6062326649505698No Hit
CGATCGATACAGCTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC3562250.5974906583222435No Hit
GATAGGTCGCATCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTG3339050.5600536690773773No Hit
GATCGATAACGCTTTTGTTGTGGAAAGGACGAAACACCGAACCCCTACCA3328680.5583143250878198No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAAACACCGAACCCCTACCAAC2505190.4201916267309429No Hit
ATCGATTCCTTGCTTCATGTGGAAAGGACGAAACACCGAACCCCTACCAA2338240.3921893625981901No Hit
ATACTGTATCTCATGTCGAAAGGACGAAACACCGAACCCCTACCAACTGG2327120.3903242222738043No Hit
TAACAATGGTCTAGTGCAAAGGACGAAACACCGAACCCCTACCAACTGGT2214270.37139606709332423No Hit
ATACACGATCTCATGTCGAAAGGACGAAACACCGAACCCCTACCAACTGG1886830.3164750645918054No Hit
GATCGATAACGCTTTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA1739430.2917518915869072No Hit
ACGATCGATAGGAAAGCTCTTGTGGAAAGGACGAAACACCGAACCCCTAC1582680.26546045760781767No Hit
GATCGATAACGCATTTGTTGTGGAAAGGACGAAACACCGAACCCCTACCA1431760.24014688047145918No Hit
TAAGTAGAGTCTAGTGCAAAGGACGAAACACCGAACCCCTACCAACTGGT1370430.22986009485144285No Hit
CGATCATGATCGACTTCTGGAAAGGACGAAACACCGAACCCCTACCAACT1346960.22592350821355298No Hit
CGATCGATACAGCTATACTTGTGGAAAGGACGAAACACCGAACCCCTACC1267690.21262767426444662No Hit
ATCGATTCCTTGGTTCATGTGGAAAGGACGAAACACCGAACCCCTACCAA1034340.173488241288239No Hit
GATCGCGCGGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTG1033600.1733641222378752No Hit
ATCGATTCCTTGNTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA764010.12814621036276996No Hit
TAACAATGGTCTNGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT759300.127356209380049No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCCAACCCCTACCAA746050.12513380746475117No Hit
ATACTGTATCTCNTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG729130.12229584215102743No Hit
ATACTGTATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCAACTGG713050.11959876873231126No Hit
GATAGGTCGCATNTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG609280.10219358784548432No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCTTG10359700.0145.868049
TCGCATC4801950.0145.75997
TGGTCTA1091000.0145.536427
TCCTTGG5620850.0145.46127
CGCATCT4850650.0145.178838
GATCGTC3388700.0145.161858
CCTTGGT5649850.0144.900928
ATAGGTA3669600.0144.895438
TAACGCA2692850.0144.711617
TGATCGT3363250.0144.707177
ATACTGT6595450.0144.51111
GGTCGCA5862950.0144.509545
ATACACG5344450.0144.498111
TGTATCT6696100.0144.435825
TACACGA5349250.0144.332382
ATCGATT6594550.0144.221981
GATCGAC609100.0144.20728
CGCATGT1046500.0144.161778
GTCGCAT5882250.0144.029246
ATGGTCT6494050.0144.021626
Produced by FastQC (version 0.11.5)