FastQCFastQC Report
Mon 7 Oct 2019
HV3CVAFXY_n01_PCR2_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3CVAFXY_n01_PCR2_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49304398
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT684395613.881025380332195No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC574986611.661973846633316No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC524806010.644202571949059No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC504436610.231067013534979No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA44195178.963737879935172No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG40328888.179570512147821No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA20147714.086392049650418No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT13025532.6418596572257105No Hit
TCGATCGTTACCTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC11323262.296602424797885No Hit
TAAGTAGAGTCTAGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT10233972.075670815410828No Hit
ACGATCGATAGGAAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC7414951.5039124907275006No Hit
GATCGATAACGCTTTTGTTGTGGAAAGGACGAAACACCGAACCCCTACCA6568871.3323091380205068No Hit
CGATCGATACAGCTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC6352341.2883921633116786No Hit
ATACACGATCTCATGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG4896610.9931385837020057No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG4074540.8264049791257974No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAAACACCGAACCCCTACCAAC3898050.7906089838070834No Hit
GATCGATAACGCTTTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA3409600.6915407424708846No Hit
TAAGTAGAGTCTAGTGCAAAGGACGAAACACCGAACCCCTACCAACTGGT3068650.622388696440427No Hit
ACGATCGATAGGAAAGCTCTTGTGGAAAGGACGAAACACCGAACCCCTAC2769420.5616983701940748No Hit
GATCGATAACGCATTTGTTGTGGAAAGGACGAAACACCGAACCCCTACCA2753460.558461336451162No Hit
ATCGATTCCTTGCTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA2484790.5039692402288332No Hit
CGATCGATACAGCTATACTTGTGGAAAGGACGAAACACCGAACCCCTACC2225300.4513390468736683No Hit
ATACACGATCTCATGTCGAAAGGACGAAACACCGAACCCCTACCAACTGG1827760.37070932292896064No Hit
CGATCATGATCGACTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT1602290.32497912255210987No Hit
ATCGATTCCTTGCTTCATGTGGAAAGGACGAAACACCGAACCCCTACCAA962070.1951286374087764No Hit
GATCGCGCGGTTGTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG861620.17475520135140887No Hit
ACGATCGATAGGTAAGCTCTTGTGGAAAGGACGAAACACCGAACCCCTAC774540.15709349092955155No Hit
CGATCGATACAGGTATACTTGTGGAAAGGACGAAACACCGAACCCCTACC768490.15586641986785843No Hit
ACGATCCATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC625800.12692579676157895No Hit
TCGATCGTTACCTTCTAGTGGAAAGGACGAAACACCGAACCCCTACCAAC584470.1185431774260787No Hit
CGATCATGATCGACTTCTGGAAAGGACGAAACACCGAACCCCTACCAACT584410.11853100812629332No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCCAACCCCTACCA573660.11635067524807827No Hit
TAAGTAGAGTCTTGTGCAAAGGACGAAACACCGAACCCCTACCAACTGGT528660.10722370040903856No Hit
ATACACGATCTCTTGTCGAAAGGACGAAACACCGAACCCCTACCAACTGG519760.10541858760753961No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACACG5186800.0144.691021
TCGTTAC7710850.0144.611015
ATCGTTA7713000.0144.601724
GATCGTT7709050.0144.564033
GTAGAGT9135500.0144.553594
ACGATCG7511300.0144.525311
CGATAGG7634650.0144.477686
TACACGA5198250.0144.39792
CGATTCC2773250.0144.344363
GTTACCA6311350.0144.325977
CGTTACC7724500.0144.266276
GATACAG6748500.0144.20686
ATAACGC6470100.0144.19066
TCGATCG7668050.0144.165191
TCGATAA6450300.0144.125213
TAGGTAA6577800.0144.105139
CGATCGT7675400.0144.0952
GATAACG6460950.0144.082785
TTACCAT6382700.0144.044258
TAGAGTC9199200.0144.027945
Produced by FastQC (version 0.11.5)