FastQCFastQC Report
Mon 7 Oct 2019
HV3CVAFXY_n01_PCR2_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV3CVAFXY_n01_PCR2_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1045
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT14413.779904306220095No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC11711.196172248803828No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC11410.909090909090908No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC10510.047846889952153No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA938.899521531100477No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG928.803827751196172No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA413.923444976076555No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT373.5406698564593304No Hit
TCGATCGTTACCTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC252.3923444976076556No Hit
TAAGTAGAGTCTAGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT171.6267942583732056No Hit
CGATCGATACAGCTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC171.6267942583732056No Hit
ACGATCGATAGGAAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC161.5311004784688995No Hit
GATCGATAACGCTTTTGTTGTGGAAAGGACGAAACACCGAACCCCTACCA100.9569377990430622No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG90.8612440191387559No Hit
ACGATCGATAGGAAAGCTCTTGTGGAAAGGACGAAACACCGAACCCCTAC70.6698564593301436No Hit
ATACACGATCTCATGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG70.6698564593301436No Hit
TCGATCGTTACCATCTAGTGGAAAGGACGAAACACCGAACCCCTACCAAC60.5741626794258373No Hit
ATCGATTCCTTGCTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA50.4784688995215311No Hit
CGATCGATACAGCTATACTTGTGGAAAGGACGAAACACCGAACCCCTACC50.4784688995215311No Hit
ATCGATTCCTTGCTTCATGTGGAAAGGACGAAACACCGAACCCCTACCAA50.4784688995215311No Hit
GATCGATAACGCATTTGTTGTGGAAAGGACGAAACACCGAACCCCTACCA40.3827751196172249No Hit
TAAGTAGAGTCTAGTGCAAAGGACGAAACACCGAACCCCTACCAACTGGT40.3827751196172249No Hit
GATCGATAACGCTTTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA40.3827751196172249No Hit
CGATCATGATCGACTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT40.3827751196172249No Hit
CGATCATGATCGACTTCTGGAAAGGACGAAACACCGAACCCCTACCAACT40.3827751196172249No Hit
ACGATCCATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC30.28708133971291866No Hit
AGGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC30.28708133971291866No Hit
ATACACGATCTCATGTCGAAAGGACGAAACACCGAACCCCTACCAACTGG30.28708133971291866No Hit
GATCGCGCGGTTGTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG20.19138755980861244No Hit
ATCGATTCCTTGCTTCTTGTGGAAAGGACGAAACACCCAACCCCAACCAA20.19138755980861244No Hit
TCGATCGTTACCTTCTTGTGGAAAGGACGAAACACCGAACCCCTATCAAC20.19138755980861244No Hit
GATCGATAACTCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA20.19138755980861244No Hit
TTAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT20.19138755980861244No Hit
ATACACGATCTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAACTGG20.19138755980861244No Hit
ACGATCGATAGGTAAGGTCTTGTGGTAAGGACGAAACACCGAACCCCTAC20.19138755980861244No Hit
AAACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG20.19138755980861244No Hit
CGATCGATACAGGTATTCTTGTGGTAAGGACGAAACACCGAACCCCTACC20.19138755980861244No Hit
TAGGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT20.19138755980861244No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGAGT251.6339982E-8145.04
AAGTAGA251.6339982E-8145.02
ACACGAT100.0060729836145.03
ATCTCTT100.0060729836145.08
CGATAGG100.0060729836145.06
CGATACA158.971295E-5145.05
CGATAAC100.0060729836145.04
TACAGGT100.0060729836145.08
CGTTACC201.2464734E-6145.06
TCGATCG201.2464734E-6145.01
TCGATAG100.0060729836145.05
TCGATAC158.971295E-5145.04
TCGATAA100.0060729836145.03
TTACCAT158.971295E-5145.08
GATACAG158.971295E-5145.06
ACGCATT100.0060729836145.09
AGTAGAG251.6339982E-8145.03
TAAGTAG251.6339982E-8145.01
ATAGGTA158.971295E-5145.08
TAACGCA100.0060729836145.07
Produced by FastQC (version 0.11.5)