FastQCFastQC Report
Mon 21 Dec 2020
HV33TBGXG_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV33TBGXG_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30977507
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG943640.30462102712138844No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG1304150.066.097311
ACACGTC1272050.066.0489413
CACACGT1303550.066.0391412
ACGTCTG1271100.065.8652915
CGTCTGA1270250.065.7686616
CACGTCT1269550.065.691514
AGCACAC1331550.065.4702110
GAACTCC1279400.065.4029921
CTGAACT1278550.065.1640319
AGAGCAC1360950.064.968278
TGAACTC1298900.064.9275720
CTCCAGT1223500.064.8206824
CCAGTCA1119500.064.2177326
AACTCCA1305450.064.17377522
CAGTCAC1031000.063.8429227
TCGGAAG1402050.063.4717333
CGGAAGA1410350.063.454634
GATCGGA1390550.063.0809971
ATCGGAA1424600.062.564082
AGTCACC324200.061.66641628
Produced by FastQC (version 0.11.5)