FastQCFastQC Report
Mon 21 Dec 2020
HV33TBGXG_n01_AKM183.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV33TBGXG_n01_AKM183.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15230868
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCCTTTATCTCGTAT9055685.945609928468949TruSeq Adapter, Index 27 (97% over 44bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG176400.11581743075969143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1003050.067.21786546
TATGCCG995000.067.0609748
GTATGCC1006650.067.0422747
ATGCCGT998450.066.8537949
TGCCGTC998500.066.7838450
TCGTATG1007150.066.6859245
CTCGTAT1008700.066.6257144
GCCGTCT988550.066.6241151
TCTCGTA1022000.065.8787443
CCTTTAT1032700.065.6702337
CCGTCTT1007150.065.5570552
CTGCTTG943050.065.4538659
ATCTCGT1042800.065.0161542
CAGTCAC1089750.064.9306927
TCCTTTA1046800.064.89439436
AGTCACA1089450.064.5347528
CTTTATC1052650.064.4918738
GTCACAT1084750.064.3623729
TGCTTGA966750.064.2466760
CCAGTCA1103200.064.0694926
Produced by FastQC (version 0.11.5)