FastQCFastQC Report
Mon 21 Dec 2020
HV33TBGXG_n01_AKM182.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV33TBGXG_n01_AKM182.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14614621
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT11068897.573846766193936TruSeq Adapter, Index 25 (97% over 44bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG358860.24554861874283296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1209700.066.9874746
GTATGCC1210450.066.85503447
TATGCCG1207300.066.8191948
ATGCCGT1209200.066.67123449
TGCCGTC1210250.066.6683450
CTCGTAT1216500.066.50873644
GCCGTCT1209150.066.5060751
TCGTATG1218750.066.505645
CCGTCTT1224400.065.8512352
GTCACAC1245150.065.7861229
CTGCTTG1209100.065.60618659
TCTCGTA1236050.065.5631343
CACACTG1239600.065.5412931
CAGTCAC1271450.065.3838927
CCAGTCA1278450.064.94952426
ATCTCGT1253000.064.87543542
AGTCACA1274250.064.7794128
TGCTTGA1229500.064.6334860
ACACTGA1256400.064.6315632
TATCTCG1256300.064.5946941
Produced by FastQC (version 0.11.5)