FastQCFastQC Report
Mon 21 Dec 2020
HV33TBGXG_n01_AKM181.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV33TBGXG_n01_AKM181.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16036178
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT10964516.837358627473454TruSeq Adapter, Index 7 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG768740.47937856514189353No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1215850.067.11159546
GTATGCC1217750.066.9831147
TATGCCG1214050.066.9629848
ATGCCGT1217200.066.7615649
CTCGTAT1218700.066.6841744
CGAGTGG1225950.066.6748833
TCGTATG1222150.066.6314145
GCCGTCT1213800.066.6117851
TGCCGTC1220500.066.60132650
ACGAGTG1232150.066.5289132
GTGGATA1231400.066.1974436
CACGAGT1244100.066.08279431
GGATATC1239500.065.9828138
AGTCACG1263250.065.9706128
GTCACGA1260050.065.84421529
CCGTCTT1230550.065.8212952
GAGTGGA1240850.065.758634
AGTGGAT1243900.065.6423935
TCACGAG1259900.065.6092130
CTGCTTG1202700.065.57619559
Produced by FastQC (version 0.11.5)