FastQCFastQC Report
Mon 21 Dec 2020
HV33TBGXG_n01_AKM180.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV33TBGXG_n01_AKM180.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15202683
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT8455055.561551207770366TruSeq Adapter, Index 22 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG154580.10167942066541806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTACG940200.067.5796633
CGTACGT936750.067.5555234
CGTATGC931550.067.36072546
GTACGTA943950.067.2402835
TATGCCG923800.067.1026948
GTATGCC936450.067.0764247
ATGCCGT924000.067.0467249
TACGTAA947450.066.8547436
GCCGTCT914950.066.82339551
TGCCGTC927850.066.7991450
CTCGTAT934250.066.7704744
TCGTATG937900.066.76613645
ACGTAAT954200.066.2922337
CGTAATC950400.066.1531938
CACCGTA969750.065.983931
ACCGTAC969350.065.7704632
GTAATCT948450.065.6298839
CCGTCTT934750.065.62096452
GTCACCG985300.065.6186429
AGTCACC997400.065.33259628
Produced by FastQC (version 0.11.5)