FastQCFastQC Report
Mon 21 Dec 2020
HV33TBGXG_n01_AKM179.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV33TBGXG_n01_AKM179.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12909619
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTAT3200092.478841552179038TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGGAT675870.5235398504014719TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTTT381030.29515201029557886TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCGCGTAT250550.19408008865327475TruSeq Adapter, Index 21 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG230280.1783786183000443No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCGCGGAT215920.16725512968275827TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGGTT178660.1383929301089366TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGTGT147290.11409321994707976TruSeq Adapter, Index 21 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGGAATCTCGGGT140450.10879484514608835TruSeq Adapter, Index 21 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGT742050.064.986429
AGTCACG752200.064.8520228
CGGAATC721450.064.4196338
CGTTTCG735800.064.3854133
GTTTCGG735550.064.34090434
TGCGTGA248900.063.7028960
CAGTCAC771950.063.53336327
CTCGTAT369350.063.4893244
GCGTGAA268900.063.4815961
TCACGTT761700.062.93577230
CGTATGC254900.062.91866346
GGAATCT594850.062.89267339
CTGCGTG214500.062.88884459
ACGTTTC756650.062.80432532
TCGTATG327950.062.7553145
CCAGTCA782850.062.5698426
AATCTCG556350.062.1750941
GTATGCC262050.062.15173747
ATGCCGT251500.062.10181849
TATGCCG240650.062.05036548
Produced by FastQC (version 0.11.5)