FastQCFastQC Report
Mon 21 Dec 2020
HV33TBGXG_n01_AKM178.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV33TBGXG_n01_AKM178.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15583640
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT10181836.533666075448355TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCTTA1133600.068.0954336
CGTATGC1131650.067.634946
GCCTTAT1144050.067.5175937
TATGCCG1122950.067.4481448
GTATGCC1138050.067.3685647
ATGCCGT1122700.067.33909649
TGCCGTC1124350.067.2124750
CTCGTAT1137050.067.19791444
TCGTATG1137300.067.1584645
CGTGGCC1158750.067.0319933
GCCGTCT1110750.067.0147951
TGGCCTT1151700.066.9499135
CCTTATC1157600.066.5441438
GTGGCCT1162050.066.425934
CTTATCT1152100.066.2440339
CCGTCTT1128000.066.21297552
TCTCGTA1159050.066.00114443
GTCACGT1207700.065.7282429
CTGCTTG1055800.065.6564259
AGTCACG1218300.065.6543928
Produced by FastQC (version 0.11.5)