FastQCFastQC Report
Mon 21 Dec 2020
HV33TBGXG_n01_AKM177.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHV33TBGXG_n01_AKM177.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13427229
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT3883372.8921604003327865TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC468050.065.27775646
TATGCCG468050.065.0271248
ATGCCGT468350.064.93339549
GTATGCC470000.064.8827347
CTCGTAT466800.064.7414444
TGCCGTC471350.064.520150
GCCGTCT471900.064.2745551
TCGTATG474750.064.1540945
GATCTCG462600.063.8196741
CGATCTC453750.063.6935340
AGTCACG490600.063.56200428
GTCACGT487050.063.3228929
ACGATCT455550.063.1282539
CCGTCTT487150.062.4631952
AACGATC462850.062.37005638
CTGCTTG481900.061.8608959
CACGTGA493750.061.77324731
TCTCGTA488050.061.75091643
TCACGTG499100.061.42464430
CAGTCAC512500.061.11884727
Produced by FastQC (version 0.11.5)